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Fig. 9. Concentration dependence of the effects of amino acids on escapin's
antimicrobial activity. (A) Plate assay of growth inhibition. E. coli
cells were grown at 37°C on a plate in minimal medium, (M9+glucose) in the
presence of amino acids and escapin, or in hydrogen peroxide alone (without
escapin) at concentrations from 3 to 50 mmol l-1. (B) Bactericidal
assay. E. coli cells were grown in M9+glucose containing 3
x108 cells ml-1, then incubated at 37°C with
escapin (60 µg ml-1) in M9+glucose with L-lysine,
L-arginine, or L-tyrosine at 3-50 mmol l-1.
Values are means ± S.E.M.,
N=3.
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