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Fig. 8. Escapin's bactericidal effect was greatest in the enriched growth medium
and L-lysine. (A,B) E. coli and (C) Staphylococcus
aureus cells in early-log growth phase were cultured in M9-glucose medium
(for E. coli) or LB (for S. aureus), and 4 samples of equal
cell density were resuspended in different growth media in the presence of
escapin (Esc: 50 µg ml-1) or (C) buffer control. Cells were then
incubated at 37°C for 60 min. Ye, 1% yeast extract; Try, 1% Tryptone
Peptone; Lys, 50 mmol l-1 L-lysine; Arg, 50 mmol
l-1 L-arginine; His, 50 mmol l-1
L-histidine; Val, 50 mmol l-1 L-valine; aa,
amino acid mixture containing 20 L-amino acids each at 50 µmol
l-1. In a follow-up experiment, a 10 times higher concentration of
Ye or aa was used, and similar results were observed. Values are means
± S.E.M., N=3. An asterisk
indicates a significant reduction in the number of viable clones
(P<0.05, t-test).
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