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Fig. 6. Escapin preferentially kills E. coli cells in their growing but
not in their resting state. 2 x108 cells ml-1 in
early-log growth or stationary phase were centrifuged, and the pellet was
resuspended in fresh LB medium. The absorbance reading was adjusted to that of
early-log phase cells (=0.5). Cells were then coincubated in escapin at 0-50
µg ml-1 at 37°C for 2 h. Cultures were then placed on LB
agar plates and incubated at 37°C for 18 h, at which time the number of
viable colonies was counted. Values are means ±
S.E.M., N=3.