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Fig. 4. LAAO enzyme activity of escapin, and its substrate specificity. 0.6 µg escapin in 100 µl was incubated at 22°C for 1 min in 2 mmol l-1 of each L-amino acid and taurine. LAAO activity was measured by absorbance at 436 nm and normalized to the value for arginine. Values are mean ± S.E.M., N=2. Inset shows a Lineweaver-Burk plot of data for LAAO activity at different concentrations for lysine and arginine. Values are means ± S.E.M., N=2. Km and Vmax values calculated from this experiment are shown.





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