spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 11. Recombinant escapin and its antibacterial activity. (A) Western blots demonstrating successful expression of recombinant escapin. (B) Plate assay of inhibition of growth of E. coli by wild-type escapin and recombinant escapin. E. coli cells in stationary phase were grown at 37°C as a lawn on LB solid medium and tested against wild-type escapin and recombinant escapin at 7-60 µg ml-1. Concentrations of wild-type and recombinant escapin were estimated from western blots. WEsc, wild-type escapin purified from ink; r-proEsc, recombinant escapin, with 18 amino acid signal peptide, in E. coli cell lysate; rEsc, recombinant in E. coli cell lysate, escapin without signal sequence (i.e. lacking 18 amino acids at N terminus); M, molecular mass markers.





Right arrow Return to article