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Fig. 1. (A) The ink of Aplysia californica containing escapin, a 60 kDa
protein. (B) SDS-PAGE of raw purple ink (left lane) and ink purified as
described in the Materials and methods to yield escapin, a 60 kDa protein
(right lane). Molecular mass standards are also shown. (C) Analytical gel
filtration shows that escapin elutes as a single peak. Absorbance is expressed
on a relative scale; mAu, milliabsorbance units. Arrow and arrowhead indicate
elution times for escapin and BSA, respectively. The inset shows elution
volumes of molecular mass standards: BSA, 67 kDa; ovalbumen, 43 kDa;
chymotrypsinogen A, 25 kDa, demonstrating that native (non-denatured) escapin
has molecular mass of ca. 60 kDa, similar to that of the denatured escapin, as
shown in B. The photograph in A is courtesy of Genevieve Anderson.
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