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Fig. 3. Visualization of hemolymph sinuses/lacunae in the commissural ganglion
(CoG) using India ink. India ink injected into the pericardial chamber is
incorporated into the circulating fluid and fills blood vessels and sinuses
with an easily visualized substrate. As this micrograph shows, the CoG is one
of the region of the nervous system possessing vascularization. Here, India
ink filling reveals an extensive array of hemolymph lacunae/sinuses within the
CoG, including many in the anterior medial quadrant of the ganglion. This
image is a digital micrograph of a living ganglion. Abbreviations:
coc, circumoesophageal connective; ion, inferior oesophageal
nerve; son, superior oesophageal nerve; SoG, supraoesophageal
ganglia; TG, thoracic ganglia. Scale bar, 200 µm.