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Fig. 7. Representative recordings of principal cell basolateral membrane voltage
(Vb) in tubules stimulated with either (A) 100 nmol
l1 Anoga-DH44 or (B) 100 nmol
l1 Anoga-DH31. The response to
Anoga-DH44 is triphasic, commencing with a transient
hyperpolarisation (Phase 1) followed by the depolarisation (Phase 2) and
repolarisation (Phase 3) of Vb before the peptide is
washed off. Horizontal bars indicate when the peptides were present in the
bath.