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Fig. 5. Sequential addition of Anoga-DH44 and Anoga-DH31 to the same batch of tubules demonstrates that only the latter has pronounced natriuretic activity. Fluid secretion and urine Na+ and K+ concentrations were measured over 30 min intervals under control conditions and after the addition of 1 µmol l–1 Anoga-DH44 alone and in combination with 1 µmol l–1 Anoga-DH31. Bars represent the means + S.E.M. for five tubules. Anoga-DH44 stimulates fluid secretion (open bars) without affecting the [Na+]:[K+] ratio (solid bars) of the secreted urine, which increases dramatically after the addition of Anoga-DH31 along with a further acceleration of urine flow. Identical letters indicate values that do not differ significantly (P>0.05).





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