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Fig. 1. Exogenous 8-bromo-cyclic AMP stimulates secretion of Na+-rich urine by An. gambiae Malpighian tubules. Fluid secretion and urine Na+ and K+ concentrations were initially measured over a 30 min control period and then at 10–15 min intervals after the addition of 1 mmol l–1 8-bromo-cyclic AMP. Data points show the means ± S.E.M. for five tubules. (A) Fluid secretion (solid line) and the tubule fluid [Na+]:[K+] ratio (broken line) increase after the addition of 8-bromo-cyclic AMP. (B) This reflects the selective stimulation of transepithelial Na+ transport (solid line) compared with K+ transport (broken line). Arrows show the time of addition of 8-bromo-cyclic AMP.





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