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Fig. 5. (A) Grey TIF-8 bit pictures from Ae. aegypti midguts were used for
image analysis. The top midgut was treated with 104 mol
l1 MTZ and Cresol Red. The lower midgut was treated with
Cresol Red in the absence of MTZ. (B) Histogram of average intensity values
for treated (104 mol l1) and untreated
midgut images (from A) analyzed with ImageQuant TL software from Amersham
Biosciences®. Differences were significant (P value<0.0001).
20 specimens (10 treated and 10 untreated) were analyzed in triplicate.