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Fig. 3. The complete sequence of calpain M cDNA from land crab (Gl-CalpM). The sequence (2531 bp) contained a full open reading frame (220–1896 bp) encoding a protein of 558 amino acids with a predicted mass of 65.23 kDa (GenBank accession #AY639152). Locations of degenerate primer sites for initial nested RT-PCR are underlined. Three amino acid residues comprising the conserved catalytic triad (C, H, N) in domain II are in bold. The asterisk indicates the stop codon. The poly(A) signal in the 3' UTR is in bold and underlined. The arrow indicates the position of a putative alternative splicing site that produces a truncated isoform (Gl-CalpM'; GenBank accession #AY644674).





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