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Fig. 2. Means (±2 S.E.M.) for (A) glycogen (expressed as glucosyl units), (B) free glucose and (C) lactate in brains of hatchling painted turtles. Values were adjusted by ANCOVA to the mean mass of 37 mg for the 61 brains examined in this study. ANCOVA for brain glycogen, F_5,54=71.23, P<0.001; for free glucose, F_5,54=25.53, P<0.001; for lactate, F_5,54=31.97, P<0.001. Treatment 0 represents controls that were not exposed to subzero temperatures; treatment 1 represents animals exposed to –6°C for 10 days; treatment 2 represents animals exposed to –6°C for 20 days; treatment 3 represents animals exposed first to –6°C for 10 days and then to –3°C for another 10 days; treatment 4 represents animals exposed first to –6°C for 10 days and then to 0°C for another 10 days; treatment 5 represents animals exposed first to –6°C for 10 days and then to +3°C for another 10 days. Bars that share the same letter cannot be distinguished statistically at P=0.05.

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