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Fig. 7. Confocal fluoroscopy of Pd-mP emission spectra in digest cells in
vitro. Digest cells were incubated for 4 h with Pd-mP-globin 100 µmol
l–1, and observed 8 h later using laser excitation at 514 nm.
Images were collected from 530 to 700 nm, with ±10 nm intervals between
each consecutive image. (A) Digest cell total emission image. Arrows indicate
areas of the image selected for spectral analysis: (1) cytosol, (2) hemosome
and (3) digest vesicle. Scale bar, 20 µm. (B) Emission spectra obtained
from image analyses. (C) Pd-mP 5 µmol l–1 fluorescence
spectra with 
ex=514 nm in water, methanol,
phosphatidylcholine liposomes, or bound to 5 µmol l–1
globin.
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