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Fig. 1. Uptake of albumin and hemoglobin by tick midgut digest cells. Digest cells
were incubated for 4 h in culture medium containing albumin or hemoglobin
labeled with rhodamine. Incubation conditions were (A,B) 1 µmol
l1 rhodaminehemoglobin; (C,D) 1 µmol
l1 rhodaminehemoglobin plus 100 µmol
l1 unlabeled hemoglobin; (E,F) 1 µmol
l1 rhodaminealbumin; (G,H) 1 µmol
l1 rhodaminealbumin plus 100 µmol
l1 unlabeled albumin. (A,C,E,G) Differential interference
contrast (DIC) images; (B,D,F,H) rhodamine fluorescence. Scale bar, 10 µm.
Large hemoglobin-accumulating vesicles (asterisks) and small
albumin-accumulating vesicles (arrows) are indicated in the figure. Hemosomes
are seen as brown-colored structures on DIC panels and are not labeled by any
of the proteins conjugated to rhodamine.