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Fig. 4. The pattern of ß2-immunoreactivity in the submucous plexus is very similar to the pattern of immunoreactivity for {alpha}3/{alpha}5-nAChRs, suggesting that these subunits are structural partners in the same heteromeric nAChRs. (A,C) Green (Alexa 488-conjugated mAb 210, 7 nmol l–1) and red (33 nmol l–1 mAb 295/TR-conjugated goat-anti-rat 1:1000) channels from a confocal micrograph taken from a submucous ganglion double-stained for {alpha}3/{alpha}5- and ß2-nAChRs. (B) Combined immunofluorescence from A and C. The upward arrow points to one of the multiple neurones that express {alpha}3/{alpha}5- as well as ß2-nAChRs. The horizontal arrow head points to a cell that expresses predominantly ß2-nAChRs. The vertical arrow head points to a cell that expresses mostly {alpha}3/{alpha}5-nAChRs. Scale bar, 50 µm.





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