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Fig. 2. The pattern of immunofluorescence obtained using mAb 306 mimics the
distribution of 
BgT-binding in submucosal ganglia, and the block of
BgT-binding by nicotine confirms that BgT reacts specifically
with enteric 7-nAChRs. (A) Submucosal ganglion stained with mAb 306 (17
nmol l–1) and FITC-conjugated goat-anti-mouse. Fixed tissue
permeabilized with Triton X-100 (0.5%). (B) Negative control for A, obtained
from the same preparation in the absence of mAb 306. (C) Binding of Alexa
594-conjugated BgT (250 nmol l–1) to a live segment of
submucosal plexus. (D) The binding of Alexa 594-conjugated BgT was
blocked by a 16 h co-incubation with 1 mmol l–1 nicotine.
Scale bar, 25 µm.
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