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Fig. 11. Myenteric neurones exhibit differential pharmacological responses to a given nAChR antagonist depending upon the location of the stimulation electrode, oral or aboral with respect to the ganglion. (A) Low-magnification image of the preparation (20x), illustrating the location of the stimulating electrodes (E₁ and E₂) with respect to the recording area. (B) High-resolution image of the myenteric ganglion of interest (inverted grey scale), showing four individual neurones identified by numbers. (C) Serial images of the selected myenteric ganglion taken at 20 min intervals throughout the experiment. Notice the changes in ganglion shape (and therefore in registration with respect to the camera) from one frame to another. The arrows have been inserted as fixed references to draw attention to the magnitude of the ganglionic movements. Magnification, 100x. (D) Stimulation protocol. (E) Experimental protocol. (F) Optical responses evoked by each of the stimulation electrodes (E₁ and E₂) according to the protocol described in D. Data are presented in two ways: signals spatially averaged over the whole ganglion (row of bars labelled `Ganglion', whose heights represent the amplitude of the voltage change evoked by each electrode, averaged over the area of interest) and signals spatially averaged over individual neurones (`Cells' numbered 1–4). (G) High-speed camera movies showing the spatial pattern of responses to stimulation by electrodes E₁ and E₂, before and after 25 min exposure to 100 nmol l^–1 -CTx MII. Frame acquisition 1 kHz. Unfiltered.

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