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Fig. 9. Developmental time course of the relative abundance of three developmental
stages dechorionated encysted embryos (open diamond), emergence and
emergence 2 stages combined (solid triangle) and nauplii (solid circle)
calculated as a percentage of all individuals present. Dechorionated
embryos incubated in 35
Instant Ocean + 0.5% ethanol served as a
control (A). Experimental treatments with 0.5 (B), 1.0 (C), 2.0 (D) and 4.0
µmol l1 (E) bafilomycin in the presence of 0.5% ethanol
began at hour 0. To determine whether or not embryos were permeable to
bafilomycin prior to emergence, 4 µmol l1 bafilomycin was
added at hour 8 (F), as indicated with a broken line.