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Fig. 5. In vivo real-time measurements of the heat shock response of
mature fowl of three strains that differ in their resistance to heat. (A) Body
temperature (Tb) regulation (N=3 in each strain).
(B) HSF–DNA-binding activity detected by EMSA. EMSA was performed with a
[32P]HSE oligonucleotide and whole blood cell extracts. (C) Levels
of HSP70–mRNA in whole blood cells, detected by chicken HSP70 cDNA as a
probe. (D) Protein levels of HSP70 detected by western blot analysis.
Leghorn-C, heat sensitive fowl; Leghorn-16d, phenotypically heat-adapted fowl;
Bedouin, genetically heat-resistant fowl. Red arrows indicate repeated
elevations of ambient temperature, in the case of the Bedouin fowl up to
43.5°C. B–D represent three individuals of each strain.
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