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Fig. 5. Expression of genes that have different patterns of expression between gills and opercular epithelium. Carbonic anhydrase 2 (CA2) (A,B) and Na+,H+-exchanger 3 (NHE3) (C,D) mRNA expression in killifish gills (A,C; black/white) and opercular epithelium (B,D; grey) after transfer from brackish water (10% seawater) to brackish water (BW, hatched bars) or freshwater (FW, solid bars) (N=7–10). Expression is relative to the expression of EF1{alpha} and is normalized to 12 h brackish water controls. Values are means ± S.E.M. *Significant difference from time-matched brackish water control (P<0.05); {dagger}significant difference from 12 h brackish water control (P<0.05).





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