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Fig. 3. Expression of genes that have similar patterns of expression between killifish gills and opercular epithelium. Na+,K+,2Cl–-cotransporter 1 (NKCC1) (A,B), cystic fibrosis transmembrane conductance regulator (CFTR) Cl– channel (C,D), and the signalling protein 14-3-3a (E,F) mRNA expression in gills (A,C,E; black/white) and opercular epithelium (B,D,F; grey) after transfer from brackish water (10% seawater) to brackish water (BW, hatched bars) or freshwater (FW, solid bars) (N=7–10). Expression is relative to the expression of EF1{alpha} and is normalized to 12 h brackish water controls. Values are means ± S.E.M. *Significant difference from time-matched brackish water control (P<0.05); {dagger}significant difference from 12 h brackish water control (P<0.05).





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