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Fig. 2. The estimated absolute expression levels of several genes important for ion transport in the gills (black bars) and opercular epithelium (grey bars) of killifish (N=15–20). Values are determined using Formula 1 and data are normalized to the expression level of elongation factor 1{alpha} (EF1{alpha}). Na+,H+-exchanger 2 (NHE2) was not amplified above background levels in opercular epithelium. NKA, Na+,K+-ATPase {alpha}1a; VHA, V-type H+-ATPase A. #Significant difference between tissues (P<0.05).





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