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Fig. 6. Double immunofluorescence microscopy of gills in tilapia adapted to freshwater (FW; A,C,E,G) and seawater (SW; B,D,F,H), stained with anti-tilapia AQP3 (A,B) and anti-Na+/K+-ATPase as a marker of chloride cells (C,D). E and F are merged images of A,C and B,D, respectively. AQP3-immunoreactive cells are observed in both FW and SW gills (A,B), and immunoreactions for AQP3 and Na+/K+-ATPase coincided completely with each other (E,F). No immunoreaction is observed in control sections (G,H). Bar, 50 µm.





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