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Fig. 2. Native gradient polyacrylamide gel showing the AFGP composition in the
gastrointestinal fluids and the serum of Arctogadus glacialis and in
the serum of Boreogadus saida. Lane 1, stomach fluid, food:
crustaceans; lane 2, mid-gut fluid, food: crustaceans; lane 3, hind-gut fluid,
food: crustaceans; lane 4, purified AFGP from Dissostichus mawsoni,
used as standard; lane 5, A. glacialis serum; lane 6, B.
saida serum; lane 7, stomach fluid, food: B. saida; lane 8,
mid-gut fluid, food: B. saida; lane 9, hind-gut fluid, food: B.
saida. All lanes were loaded with equal amount of AFGP (250 g). The main
AFGP sizes for the D. mawsoni standard are indicated. Arrow a
indicates an AFGP synthesized by A. glacialis and that is present in
all the gastrointestinal fluids; arrow b shows an AFGP in B. saida
serum that can be found in all the gastrointestinal fluids; arrow c indicates
that AFGP7 has a higher intensity than the AFGP7 in lane 1 (arrow e); arrow d
indicates that AFGP8 has higher intensity than AFGP8 in lane 1 (arrow f);
arrow g shows the increase in concentration of the high molecular mass AFGPs
in the gastrointestinal tract, which originate in the disappearance of the low
molecular mass AFGPs.
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