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Fig. 3. (A) Hepatopancreas and adnexal tissue of premolt C. magister stained with hematoxylin and eosin. Abbreviations: h, hepatopancreas tubules; b, B cells; f, F cells; r, R cells; m, muscle; rc, reserve cells in connective tissue; p, pigment granules. (B,C) In situ hybridization. Cell-specific expression of cryptocyanin and hemocyanin mRNA in hepatopancreas tissue of premolt C. magister. (B) Hemocyanin-specific antisense probes reveal that hemocyanin mRNA is expressed in R cells of hepatopancreas tubules. Hemocyanin sense probes show no reactivity. (C) Cryptocyanin-specific antisense probe shows extremely strong reactivity in R cells of hepatopancreas. Same tissue as Fig. 2B, 10 µm distant. Cryptocyanin sense probe shows no reactivity. Scale bar: A=100 µm; B,C=300 µm.





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