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Fig. 4. SWS1 opsins. (A) Deduced amino acid sequences from six species of
notothenioid fish (GenBank accession numbers AY927651-6). The Phe residue at
the equivalent site to 86 in the bovine opsin sequence that is critically
important for tuning into the UV is boxed, together with the counterion Glu at
the equivalent site to 113 and the Lys at the equivalent site to 296 that
binds the chromophore. (B) In vitro absorbance spectrum for the
pigment from D. mawsoni. Recombinant SWS1 opsin was produced in
transfected HEK 293T cells and the pigment regenerated with
11-cis-retinal. The difference spectrum shown with a fitted
Govardovski visual pigment template, was obtained by subtracting the
acid-denatured spectrum from the dark spectrum.
