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Fig. 6. (A–E) UV opsin-ir in the retina and optic lobe (Cy3, red). Nuclei
were stained with DAPI (blue). (A) UV opsin-ir of the most proximal part of
the lamina (C-layer), adjacent to the first optic chiasm (arrow). (B) Adjacent
section to A processed with the primary antibody omitted. Strong
autofluorescence of the cuticle and trachea are indicated here and in C by
arrowheads. (C) UV opsin-ir in the retina and the lamina. The width of the
labeled neuropil increases towards the lateral edges of the lamina (bottom
left). (D) Perikarya cluster at the ventral edge of the lamina (arrow). The
maximum number of labeled cells we could detect in a single section was 15.
(E) The column-like structure of the UV opsin-ir neuropil in the proximal part
of the lamina. Two layers of nuclei border the labeled neuropil (blue). The
structure of UV opsin-ir columns found in our study shows high similarity with
the optical cartridges described at the same location in the honeybee by
Sinakevitch et al. (2003)
using reduced silver staining. Arrow indicates cell layer comprising glial
nuclei or nuclei of the monopolar cells that communicate with the long
photoreceptor cell fibers. (F) UV opsin mRNA detected by in situ
hybridization using an antisense riboprobe. Hybridization signal was found in
the perikarya of the two layers bordering the lamina columns (see also arrow
in E) in between which the UV opsin-ir was detected and in perikarya at the
distal rim of the medulla (arrowhead), adjacent to the first optic chiasm.
(Inset) PER-like-ir (green) was found in the perikarya layer that is located
adjacent to the distal rim of the medulla in the first optic chiasm and in the
3rd layer of the lamina (arrow). Nuclei are stained with DAPI (blue). Arrows
in F and in the inset indicate similar location. D, dorsal; 1. Oc, first optic
chiasm; La, lamina; Me, medulla; Re, retina; V, ventral. Scale bars, 50 µm
(A,B,C,F); 20 µm (D,E).
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