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Fig. 5. UV opsin-ir in the dorsal ocelli of a worker bee (Alexa Fluor 488; green).
Nuclei were stained with DAPI (blue). (A) Tissue was fixed for 45 min at
4°C and then 20 min at room temperature in 4% paraformaldehyde before
being sectioned on a cryostat. Sections (12 µm) were incubated with a 0.023
nmol ml–1 dilution of the anti-UV opsin antibody. Arrows
indicate UV opsin-ir rhabdomeres in some but not all photoreceptor cells in
both ocelli. The median ocellus is on the left and one of the lateral ocelli
is on the right. Electron microscopy studies of the dorsal ocelli in the
worker bee, Apis mellifera, have previously identified both planar
and non-planar rhabdoms (Toh and Kuwabara,
1974). The arrow on the right points to a planar-like rhabdom, and
on the left to a non-planar rhabdom. The rhabdoms lie directly beneath a layer
of corneagenous cell nuclei (cn). The cell bodies of the retinular cells
subtend the rhabdomeres and are filled with pigment granules (pg) (not shown).
Arrowheads indicate strongly autofluorescing cuticle. (B) Adjacent section
incubated with 0.023 nmol ml–1 dilution of the anti-UV opsin
antibody mixed for 30 min with 2.3 nmol ml–1 peptide prior to
application to the slides and parallel processing with the section shown in A.
Arrows indicate the location of the rhabdomere layer, where the UV opsin
protein immunoreactivity has been abolished. Arrowheads indicate the strongly
autofluorescing cuticle. The corneagenous cells of the ocelli, like that of
the compound eye, are themselves also autofluorescing. cn, nucleus of the
corneagenous cell; pg, pigment granule layer of retinular cells. Scale bar, 50
µm.
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