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Fig. 1. The nucleotide sequence, including the untranslated regions, and the
deduced amino acids of the UV opsin cDNA. Unbolded numbers are for
nucleotides, and bolded numbers are for amino acids. The nucleotide sequence
to which the gene-specific reverse primer binds is boxed. The location of the
degenerate primer used in 3' RACE is underlined. Grey boxes indicate the
amino acid residues that are conserved between B. impatiens and
P. glaucus within the peptide domain used to generate the anti-UV
antibody (Lampel et al., in
press). The first two boxes indicate candidate glycosylation sites
(NXS/T) (O'Tousa, 1992), and
the third box indicates the lysine (K) that confers UV sensitivity
(Salcedo, 2001;
Salcedo et al., 2003). A pair
of conserved cysteines (C) that form an essential disulfide bridge in bovine
rhodopsin (Karnik et al.,
1988) are located at amino acid positions 120 and 197 (boxed). The
site of chromophore attachment (amino acid 318;
Bownds, 1967) is also boxed. A
highly conserved motif (E/DR) involved in G-protein binding/activation
(Baldwin, 1997) is present at amino acid positions 144 and 145. GenBank
accession number: AY655163.
