Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig.·S1. UV opsin-ir (Alexa Fluor 488, green) is visible in the lamina organ and diffusely in the medulla, where the photoreceptor axons of the R1 and R5 cells are presumed to terminate (upper left panel). When the primary antibody was omitted, no staining was observed (lower left panel). Nuclei were stained with DAPI (shown in adjacent panels). Abbreviations: La, lamina; Oc, optic chiasm; Me, medulla. Scale bars, 100·mm.

• Supplemental Figure 2 -

  Fig.·S2. Peptide competition experiments showing the abolishment of UV opsin-immunoreactivity (-ir) in the retina (Re), the lamina organ (La) and the cluster of perikarya located adjacent to the lateral protocerebrum. The antibody (0.023·nmol·l^–1) in blocking solution was incubated with the peptide (2.3·nmol·ml^–1) against which it was raised for 1·h at room temperature. Afterwards, 200·ml of the solution was applied per slide and sections were incubated overnight at 4°C. In the control experiment (A,C,E) the slides were treated in exactly the same way except that the peptide was omitted. UV opsin-ir could not be detected in the (B) retina, (D) lamina or (E) perikarya on the edge of the protocerebrum and mushroom bodies when the antibody was incubated with the peptide. In the control experiments, where the peptide was omitted, (A) the rhabdomeres in the retina, (C) the lamina organ and (F) the perikarya show a strong UV opsin-ir (Alexa Fluor 488, green). Nuclei are stained with DAPI (blue). Abolishment of the UV opsin-ir was also observed in the antennal lobes and central body (data not shown). Scale bars, 50·mm. Me, medulla; Mb, mushroom body; 1. Oc, first optic chiasm.