Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig.·S1. Alignment of the membrane-spanning regions of CCA-1 and three human T-type calcium channel proteins. The positions of the transmembrane domains follow those described by Perez-Reyes et al. (1998). The sequences of the three human a₁ subunits that form T-type channels were obtained from GenBank (accession numbers: NP061496 (a_1G), AAC67239 (a_1H), and NP066919 (a_1I)). The aspartate residues that distinguish LVA from HVA channels are marked with asterisks. The regions deleted in the ad1650 and gk30 alleles are indicated.

• Supplemental Figure 2 -

  Fig.·S2. A phylogenetic comparison of CCA-1 with the complete complement of predicted voltage-sensitive calcium channel a₁ subunits from C. elegans, human and Drosophila. All sequences were obtained from GenBank (accession numbers: NP000059 (human a_1A), NP00709 (human a_1B), NP000710 (human a_1C), L15453 (rat a_1E), P91645 (Drosophila a_1A/DrosM CA1A), Q24270 (Drosophila a_1C/DrosM CA1D), AAP13107 (UNC-2) and NP741442 (EGL-19)).

• Supplemental Figure 3 -

  Fig.·S3. Extrapharyngeal expression of cca-1. All images show L3 stage transgenic animals of strain TS33. These animals carry the pcca-1::GFP construct in which GFP has been fused in-frame to the third exon of cca-1 (see Materials and methods for complete details). (A) A typical transgenic animal showing GFP expression in the anterior-most body wall muscles (bwm, labeled with asterisks), neurons within the head region and pharynx (large arrow), distal tip cells (dtc), a subset of motor neuron cell bodies located in the ventral nerve cord (vnc), and cells of the intestine, consistently the most posterior cells (int). (B) Expression of pcca-1::GFP in the distal tip cell (dtc). (C) Closer examination of the head region shows pcca-1::GFP expression in the pharyngeal neurons I1, MC and M4. The anterior-most body wall muscles are again indicated by the asterisks. (D) Expression of pcca-1::GFP in the tail region including intestinal cells (int), and the muscle cell (mu bod). The arrow indicates the position of the anus.