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Fig. 4. Spatiotemporal expression of HvAPX1 and ascorbate-dependent peroxidase activity in hermaphroditic H. viridis. (A-C) Whole-mount in situ hybridisation. (D,E) Cellular in situ hybridisation. (D) Early oocyte; (E) cells from the ovary region showing HvAPX1 expression in one of the interstitial cells. (F-I) Peroxidase activity in the absence (F) or presence of ascorbate as competing substrate at 1 mmol l-1 (G), 500 µmol l-1 (H), and 200 µmol l-1 (I). Scale bars: 100 µm (D) and 5 µm (E).





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