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Fig. 1. Anatomical relationship between the excitor (Calcium Green-1) and inhibitor (Alexa 568; red) axons on the surface of the crayfish opener muscle. (A) The main Y branch of the excitor and inhibitor axons, showing how the axons project in parallel across the surface of the muscle. (B) The axons branch to form varicose terminal boutons. (C) The complex anatomical relationship between excitor and inhibitor terminals at the level of magnification used to image the Ca influx into excitor terminals. (D) Schematic of the anatomy of the excitor (green) and inhibitor (red) axons showing branching (overlap yellow). Scale bars: 200 µm (A,D), 50 µm (B) and 10 µm (C).





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