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Fig. 4. (A) CnA nuclear localisation was significantly higher in the E15 and E30 groups relative to the TR group (P<0.001, one-way ANOVA; **P<0.01, ***P<0.001, Tukey's test). Overall CnA concentration in fast muscle tissue cellular extracts did not vary between the three experimental groups. (B) CnB nuclear localisation was significantly higher in the E15 and E30 groups relative to the TR group (P<0.01, Kruskal Wallis; *P<0.05, aP<0.1, post-hoc multiple comparisons). Overall CnB concentration in fast muscle tissue cellular extracts was invariant between the three experimental groups. (C) NFAT2 nuclear localisation was significantly reduced in the E15 and E30 groups relative to the TR group (P<0.001, one-way ANOVA; **P<0.01, ***P<0.001, Tukey's test). Overall NFAT2 concentration was significantly lower in the E15 and E30 groups relative to the TR group (P<0.001, one-way ANOVA; ***P<0.001, Tukey's test). Data are mean group optical density (± S.E.M.) expressed as a percentage of the TR group. TR and E15, N=9; E30, N=6.





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