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Fig. 8. Whole mounts showing the distribution of NADPH diaphorase staining in
autozooid polyps of R. koellikeri. (A,B) Overview at two different
focal planes of the upper part of a polyp where the base of tentacles is
heavily populated by brown endosymbiotic algae. Note that focus is on staining
of the oral disc in A, and on aboral tentacle and body column staining in B
where bundles of tentacle neurites (arrows) extend to the body column
(arrowheads). (C) Enlarged view of an oral disc region corresponding to the
rectangle in A. Note clusters of cell somata at the margin (arrowheads) and
meshwork of cell processes throughout the oral disc (arrow). (D) Enlarged view
of a tentacle area corresponding to the rectangle in B. Note neurites running
parallel to each other and cell somata (arrows) at the tentacle/body column
interface. (E) Two bands of basiectodermal stained cell somata flank a
tentacle on the aboral side. Processes from these cell bands extend towards
the endodermal layer of the tentacle (arrow) and in the septum endoderm of the
column (arrowhead). (F) Bands of stained neurites on the oral side of a
tentacle (arrows) appear to merge into the similarly stained oral disk
nerve-net. (G) Enlarged view of stained basiectodermal cell somata with an
apical process (arrowheads). Neuritic processes from these cells (between
arrows) extend towards the ectoderm/mesoglea interface. Scale bars: 200 µm
(A,B), 25 µm (C), 30 µm (D), 80 µm (E), 50 µm (F) and 15 µm
(G).
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