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Fig. 1. Arrangement of the Doppler flow probe () and vascular occluder (VO) on the ventral aorta, and catheters for blood pressure measurements in sinus venosus (PCV) and the efferent branchial artery (PDA). Close to the slit in the cuff of the occluder a hole was drilled using a 20-gauge injection needle. The luminal side of the hole was countersunk using a dental drill. The catheter (a) was pulled from the luminal side with the rubber facing the lumen. Inflation of the rubber thus occluded the aorta located inside the cuff (b). This VO is similar to the one used on trout by Zhang et al. (1998).





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