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Fig. 1. Schematic representation of mWap65-1-hrGFP5k and mWap65-2-hrGFP5k constructs. (A) Structures of mWap65-1 and mWap65-2. The initiation methionine codon (ATG) of mWap65-1 and mWap65-2 is located at 29 bp and 89 bp downstream the putative transcription start point, respectively. Promoters used in this study were DNA fragments of 5 kb in the 5'-flanking region for both mWap65s. Black boxes and lines indicate exons and introns, respectively. Numbers under the boxes indicate exon numbers from the 5' end. (B) Structures of mWap65-1-hrGFP5k and mWap65-2-hrGFP5k constructs. DNA fragments of 5 kb in the 5'-flanking regions for both mWap65s were inserted into the phrGFP vector. pA, polyA signal.





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