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Fig. 1. Example records of fused isometric tetanus of an isolated muscle fiber of
the mouse. (A) Standard `isometric' recording (fixed fiber ends). (B) Force
production during length clamp of a fiber segment. Upper trace in each panel
shows force; lower trace, segment length recording with sarcomere spacing
indicated. A downward deflexion of segment length signal indicates shortening.
An initial transient at the onset of the length-clamp manoeuvre in B caused
the early tension step. Note, however, that the time required to reach plateau
force is quite similar during contraction with fixed fiber ends and during
segment length-clamp recording. The intrinsic shortening during the standard
isometric recording in A was 5.0% of fibre length. Temperature,
23.5°C.
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