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Fig. 4. Hydrolytic activity of Na+/K+ ATPase after exposure to different oxygen concentrations and manipulation of cellular redox state. Cells were exposed for 15 min to 21, 5 or 1% O2 in the presence or in the absence of 1 mmol l-1 CDNB, 5 mmol l-1 N-acetyl cysteine or GSH. After incubation, the cells were immediately destroyed by repetitive freezing-thawing and ouabain-sensitive production of Pi measured in cell homogenate in the presence of 3 mmol l-1 ATP. Data are means ± S.E.M. of five independent experiments. *P<0.05.





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