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Fig. 1. Changes in H2O2 levels in trout hepatocytes in response to hypoxia, MPG and CDNB. (A) Original traces of the changes in intensity of DCF fluorescence from individual cells over time. (B) Average changes in the rate of increase in fluorescence during the first 300 s and an interval of 800 to 1100 s of recording. Onset of hypoxic exposure or addition of 5 mmol l-1 MPG or 1 mmol l-1 CDNB corresponded to the 400 s time point. When analyzed, data for the corresponding intervals of time were fitted using linear regression and slope of each curve used to characterize increase in fluorescence for single cells with time. Presented values are means ± S.E.M. for 28-35 individual cells. * indicates P<0.05.





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