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Fig. 4. (A) Representative scan showing spatial and temporal heterogeneity of
salicylate flux at seven locations along the main segment of the
Drosophila Malpighian tubule. At each location, the position of the
Sal-SeR microelectrode at the epithelial surface is indicated by the arrowhead
of the left-most arrow. The arrows corresponding to 11 additional scans made
at intervals of 10 min at the same seven sites are indicated by the adjacent
arrows, which have been offset to the right for clarity. ASET software
calculated the salicylate-specific signal differences between the two limits
of microelectrode excursion by subtracting the voltage at the outer limit of
the excursion from that measured at the inner limit. The length of each arrow
corresponds to the mean differential signal based on three measurements at
each time interval. (B,C) Plots of differential signal of the Sal-SeR
microelectrode as a function of time at four sites in the main segments of two
Malpighian tubules. The positions of sites 2-4 relative to site 1 are
indicated in the legend. Each point is the mean of three measurements.
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