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Fig. 1. Colorimetric scoring of hemoglobin content of daphnids. Criteria used to
assign a color score to individual daphnids are listed in
Table 1. Animals that were
scored 9 were placed on a color grid of progressively darker shades of copper
and a score was assigned based upon the best color match with the grid.
Example relationships between color score assigned to daphnids and hemoglobin
content normalized to the soluble protein content of the daphnids (A) and as
total hemoglobin per daphnid (B). Values are means ±
S.E.M.(N=2-4).