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Fig. 2. Overview of the experimental conditions in progressive stages of
dissection. Note that images do not show the position of the rat hind limb in
the experimental set-up. (A) Initial condition. Dorsal view of the
lower hind limb of the rat after the GM and GL have been separated. Below the
GM and GL, the PL is visible. The distal tendons of the GM and PL were the
only tendons connected to the calcaneal bone. The tip of a pair of tweezers is
shown in the image, pointing at the GM and PL tendons. The cut calcaneal bone
was connected to a force transducer (not shown). The dissected proximal origin
of GM is not visible in this image. SN indicates the sciatic nerve. (B) Post
PL-tenotomy. Dorsolateral view. A very small area of the connective tissue
(barely visible but indicated by an arrow) was dissected to perform the
PL-tenotomy. (C) Connective tissues around the GM muscle belly. Medial view.
After dissection of the epitendinous tissues, the GM muscle belly is still
connected to extramuscular tissue (i.e. remnants of the general fascia and
epimysium). These tissues, holding the muscle belly, are indicated by an
arrow. (D) Full dissection of the GM muscle. Ventral view. The GM muscle belly
has been dissected free from its extramuscular tissues except for the
neuro-vascular tract (i.e. nerves, blood vessels and the connective tissue).
The arrow indicates the femoral artery entering the GM proximally and
medially; the nerve is entering GM proximally and laterally and is therefore
not visible in this image. In this view, the dissected GM origin, with a piece
of the femur, is visible.
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