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Fig. 2. (A) In situ hybridization expression patterns of CYP1A mRNA in
juvenile lake trout midbrain of control fish and fish exposed to
ß-naphthoflavone (BNF) for 4 days. CYP1A mRNA-positive cells are denoted
by blue stain. Control fish were injected with corn oil vehicle daily for 4
days before sacrifice. Scale bars, 100 µm. (B) Higher magnification of
BNF-exposed fish midbrain showing blue-stained glial cells (black arrows) and
endothelia (gray arrow). Scale bar, 10 µm. Horizontal sections were
counterstained with nuclear Fast Red.
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