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Fig. 3. Relationships between the duration of the exposure to –5°C and the volume of haemolymph sample (A), the loss of fresh mass (FM) (inset) and the hydration of fat body cells (DM, dry mass) (B) in the adults of Pyrrhocoris apterus belonging to three different acclimation groups (LD, SD, SDA; see Fig. 1). In A, each data point represents a mean ± S.E.M. of 6 samples (LD) or 3 samples (SD, SDA), each consisting of pooled haemolymph taken from 10 insects. The probabilities that slopes of linear regressions deviate from zero were: LD, P<0.001; SD, P=0.469; SDA, P=0.136. In the inset, each data point represents a mean ± S.E.M. of 30 individuals weighed prior to and after the exposure to –5°C. In B, each data point represents a mean ± S.E.M. of 6 samples (LD) or 3 samples (SD, SDA), each consisting of pooled fat body tissues taken from 10 insects. The probabilities that slopes of linear regressions deviate from zero were: LD, P=0.567; SD, P=0.204; SDA, P<0.001.





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