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Fig. 6. SDS-PAGE (left) and immunoblot (right) analyses using purified fluorescing proteins from the native gel shown in Fig. 5. Lane 1, Papilio xuthus; 2, Papilio machaon; 3, Papilio protenor; 4, Papilio helenus; 5, Graphium sarpedon; 6, Vanessa indica; 7, Pieris rapae. Except for Vanessa indica, the purified fluorescing proteins appeared as single bands. The molecular masses of these proteins were between 25 kDa and 31 kDa, all of which were detected by the anti-Papilio RBP. In Vanessa indica, only the 25 kDa protein was detected by anti-Papilio RBP.





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