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Fig. 8. Effect of 5-hydroxytryptamine (5-HT) and ionomycin on [Ca2+]i in scattered single ciliary cells. Scattered single ciliary cells were microinjected with fura-2 dextran and imaged once every 15 s. (A) A representative trace of an isolated tuft cell that did not respond to 100 µmol l–1 5-HT and exhibited only a weak increase in [Ca2+]i in response to 10 mmol l–1 ionomycin. (B) A representative trace of an isolated tuft cell that displayed oscillations in [Ca2+]i. Dotted lines represent the time when the specified treatments were initiated.





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