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Fig. 5. Effect of 5-hydroxytryptamine (5-HT) and ionomycin on ciliary beat
frequency (CBF) in pedal, dorsolateral and scattered single ciliary cells.
Perfusion with 100 µmol l–1 5-HT resulted in an
approximate doubling in the rate of ciliary beating in pedal (A), fura
dextran-loaded pedal (B) and dorsolateral (C) ciliary cells. This
5-HT-stimulated increase in CBF was statistically significant in all cases.
The CBF partially recovered when cells were perfused for 5 min with
Helisoma saline (HS), whereas perfusion with 10 mmol
l–1 ionomycin further stimulated CBF in pedal (A), fura
dextran-loaded pedal (B) and dorsolateral (C) ciliary cells. The scattered
single ciliary cells did not display cilio-excitatory responses to 5-HT or
ionomycin (D). Measurements were taken once every minute, N=6 cells
for A, C and D and N=4 cells for B. Dotted lines represent the time
when the specified treatments were initiated.
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