|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Quantitative RT-PCR analyses on mRNAs encoding mWap65-1 and mWap65-2, and
ß-actin from embryos at various developmental stages (h.p.f.). (A)
Agarose gel electrophoresis patterns for amplified products obtained by
RT-PCR. Total RNAs were isolated from embryos at different developmental
stages (see Table 1). The arrow
indicates the stage at which the liver begins to develop. After PCR, amplified
products were separated on agarose gels and stained with ethidium bromide.
Numbers in the left margin represent base pairs of 
X174 DNAs digested
with HincII. (B) Changes in relative transcription levels of
mWap65-1 and mWap65-2 during ontogeny. The signal
intensities of each transcript in agarose gels stained with ethidium bromide
were quantified, using Electrophoresis Documentation and Analysis System 120,
relative to those of ß-actin at the same stage.
|
